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ebss medium  (InvivoGen)
96
InvivoGen ebss medium
RyR3 deficiency induces autophagy defects A. Quantification of control and patients primary myoblasts cell death under basal culture conditions and 4h starvation <t>(EBSS).</t> *p < 0.05, ***p < 0.001, ****p < 0.0001, ns , non-significant from 2-way ANOVA and Sidak’s post hoc test. B-C. Representative images ( B ) and quantification ( C ) of LC3 positive vesicles in control and patients’ primary myoblasts cultured in basal or 2h starvation conditions. ****p < 0.0001, ns , non-significan from two-way ANOVA followed by Sidak’s post hoc test. D-E. Representative western blotting ( D ) and quantification ( E ) of LC3-II in control and RYR3 patients’ primary myoblasts starved (EBSS) or incubated with the autophagosome-lysosome fusion <t>inhibitor</t> <t>bafilomycin</t> A1 (Baf) for indicated time points. Data are represented as mean + SD, *p < 0.05, **p < 0.01, ****p < 0.0001 from two-way ANOVA and Bonferonni post-hoc test. F-G. Representative western blotting ( F ) and quantification ( G ) of LC3-II in control and RYR1 patients primary myoblasts starved (EBSS) or incubated with bafilomycin A1 (Baf) for indicated time points. Data are represented as mean + SD *p < 0.05, **p < 0.01, ****p < 0.0001 from two-way ANOVA and Bonferonni post-hoc test. H-I. Confocal imaging of skeletal muscle ( H ) and autophagy index quantification of the normalized RFP to GFP signal ( I ) of larvae co-injected with the autophagic GFP-LC3-RFP-LC3ΔG probe and control-MO or ryr3-MO. *p < 0.05 from Student’s t-test.
Ebss Medium, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mem+ebss/med_rxiv__64898__2026__02__27__26345848-85-9-20?v=InvivoGen
Average 96 stars, based on 1 article reviews
ebss medium - by Bioz Stars, 2026-07
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minimal essential medium mem  (Cytiva Europe)
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Cytiva Europe minimal essential medium mem
RyR3 deficiency induces autophagy defects A. Quantification of control and patients primary myoblasts cell death under basal culture conditions and 4h starvation <t>(EBSS).</t> *p < 0.05, ***p < 0.001, ****p < 0.0001, ns , non-significant from 2-way ANOVA and Sidak’s post hoc test. B-C. Representative images ( B ) and quantification ( C ) of LC3 positive vesicles in control and patients’ primary myoblasts cultured in basal or 2h starvation conditions. ****p < 0.0001, ns , non-significan from two-way ANOVA followed by Sidak’s post hoc test. D-E. Representative western blotting ( D ) and quantification ( E ) of LC3-II in control and RYR3 patients’ primary myoblasts starved (EBSS) or incubated with the autophagosome-lysosome fusion <t>inhibitor</t> <t>bafilomycin</t> A1 (Baf) for indicated time points. Data are represented as mean + SD, *p < 0.05, **p < 0.01, ****p < 0.0001 from two-way ANOVA and Bonferonni post-hoc test. F-G. Representative western blotting ( F ) and quantification ( G ) of LC3-II in control and RYR1 patients primary myoblasts starved (EBSS) or incubated with bafilomycin A1 (Baf) for indicated time points. Data are represented as mean + SD *p < 0.05, **p < 0.01, ****p < 0.0001 from two-way ANOVA and Bonferonni post-hoc test. H-I. Confocal imaging of skeletal muscle ( H ) and autophagy index quantification of the normalized RFP to GFP signal ( I ) of larvae co-injected with the autophagic GFP-LC3-RFP-LC3ΔG probe and control-MO or ryr3-MO. *p < 0.05 from Student’s t-test.
Minimal Essential Medium Mem, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mem+ebss/pmc12699917-45-11-46?v=Cytiva+Europe
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minimal essential medium mem - by Bioz Stars, 2026-07
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ebss medium  (Servicebio Inc)
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Servicebio Inc ebss medium
RyR3 deficiency induces autophagy defects A. Quantification of control and patients primary myoblasts cell death under basal culture conditions and 4h starvation <t>(EBSS).</t> *p < 0.05, ***p < 0.001, ****p < 0.0001, ns , non-significant from 2-way ANOVA and Sidak’s post hoc test. B-C. Representative images ( B ) and quantification ( C ) of LC3 positive vesicles in control and patients’ primary myoblasts cultured in basal or 2h starvation conditions. ****p < 0.0001, ns , non-significan from two-way ANOVA followed by Sidak’s post hoc test. D-E. Representative western blotting ( D ) and quantification ( E ) of LC3-II in control and RYR3 patients’ primary myoblasts starved (EBSS) or incubated with the autophagosome-lysosome fusion <t>inhibitor</t> <t>bafilomycin</t> A1 (Baf) for indicated time points. Data are represented as mean + SD, *p < 0.05, **p < 0.01, ****p < 0.0001 from two-way ANOVA and Bonferonni post-hoc test. F-G. Representative western blotting ( F ) and quantification ( G ) of LC3-II in control and RYR1 patients primary myoblasts starved (EBSS) or incubated with bafilomycin A1 (Baf) for indicated time points. Data are represented as mean + SD *p < 0.05, **p < 0.01, ****p < 0.0001 from two-way ANOVA and Bonferonni post-hoc test. H-I. Confocal imaging of skeletal muscle ( H ) and autophagy index quantification of the normalized RFP to GFP signal ( I ) of larvae co-injected with the autophagic GFP-LC3-RFP-LC3ΔG probe and control-MO or ryr3-MO. *p < 0.05 from Student’s t-test.
Ebss Medium, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lad2 cells  (Cytiva Europe)
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Cytiva Europe lad2 cells
RyR3 deficiency induces autophagy defects A. Quantification of control and patients primary myoblasts cell death under basal culture conditions and 4h starvation <t>(EBSS).</t> *p < 0.05, ***p < 0.001, ****p < 0.0001, ns , non-significant from 2-way ANOVA and Sidak’s post hoc test. B-C. Representative images ( B ) and quantification ( C ) of LC3 positive vesicles in control and patients’ primary myoblasts cultured in basal or 2h starvation conditions. ****p < 0.0001, ns , non-significan from two-way ANOVA followed by Sidak’s post hoc test. D-E. Representative western blotting ( D ) and quantification ( E ) of LC3-II in control and RYR3 patients’ primary myoblasts starved (EBSS) or incubated with the autophagosome-lysosome fusion <t>inhibitor</t> <t>bafilomycin</t> A1 (Baf) for indicated time points. Data are represented as mean + SD, *p < 0.05, **p < 0.01, ****p < 0.0001 from two-way ANOVA and Bonferonni post-hoc test. F-G. Representative western blotting ( F ) and quantification ( G ) of LC3-II in control and RYR1 patients primary myoblasts starved (EBSS) or incubated with bafilomycin A1 (Baf) for indicated time points. Data are represented as mean + SD *p < 0.05, **p < 0.01, ****p < 0.0001 from two-way ANOVA and Bonferonni post-hoc test. H-I. Confocal imaging of skeletal muscle ( H ) and autophagy index quantification of the normalized RFP to GFP signal ( I ) of larvae co-injected with the autophagic GFP-LC3-RFP-LC3ΔG probe and control-MO or ryr3-MO. *p < 0.05 from Student’s t-test.
Lad2 Cells, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ebss medium  (Beijing Solarbio Science)
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Beijing Solarbio Science ebss medium
RyR3 deficiency induces autophagy defects A. Quantification of control and patients primary myoblasts cell death under basal culture conditions and 4h starvation <t>(EBSS).</t> *p < 0.05, ***p < 0.001, ****p < 0.0001, ns , non-significant from 2-way ANOVA and Sidak’s post hoc test. B-C. Representative images ( B ) and quantification ( C ) of LC3 positive vesicles in control and patients’ primary myoblasts cultured in basal or 2h starvation conditions. ****p < 0.0001, ns , non-significan from two-way ANOVA followed by Sidak’s post hoc test. D-E. Representative western blotting ( D ) and quantification ( E ) of LC3-II in control and RYR3 patients’ primary myoblasts starved (EBSS) or incubated with the autophagosome-lysosome fusion <t>inhibitor</t> <t>bafilomycin</t> A1 (Baf) for indicated time points. Data are represented as mean + SD, *p < 0.05, **p < 0.01, ****p < 0.0001 from two-way ANOVA and Bonferonni post-hoc test. F-G. Representative western blotting ( F ) and quantification ( G ) of LC3-II in control and RYR1 patients primary myoblasts starved (EBSS) or incubated with bafilomycin A1 (Baf) for indicated time points. Data are represented as mean + SD *p < 0.05, **p < 0.01, ****p < 0.0001 from two-way ANOVA and Bonferonni post-hoc test. H-I. Confocal imaging of skeletal muscle ( H ) and autophagy index quantification of the normalized RFP to GFP signal ( I ) of larvae co-injected with the autophagic GFP-LC3-RFP-LC3ΔG probe and control-MO or ryr3-MO. *p < 0.05 from Student’s t-test.
Ebss Medium, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mem+ebss/pmc12573833-85-13-15?v=Beijing+Solarbio+Science
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ebss medium - by Bioz Stars, 2026-07
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cm10040 mem ebss l glutamine macgene  (Beijing Solarbio Science)
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Beijing Solarbio Science cm10040 mem ebss l glutamine macgene
RyR3 deficiency induces autophagy defects A. Quantification of control and patients primary myoblasts cell death under basal culture conditions and 4h starvation <t>(EBSS).</t> *p < 0.05, ***p < 0.001, ****p < 0.0001, ns , non-significant from 2-way ANOVA and Sidak’s post hoc test. B-C. Representative images ( B ) and quantification ( C ) of LC3 positive vesicles in control and patients’ primary myoblasts cultured in basal or 2h starvation conditions. ****p < 0.0001, ns , non-significan from two-way ANOVA followed by Sidak’s post hoc test. D-E. Representative western blotting ( D ) and quantification ( E ) of LC3-II in control and RYR3 patients’ primary myoblasts starved (EBSS) or incubated with the autophagosome-lysosome fusion <t>inhibitor</t> <t>bafilomycin</t> A1 (Baf) for indicated time points. Data are represented as mean + SD, *p < 0.05, **p < 0.01, ****p < 0.0001 from two-way ANOVA and Bonferonni post-hoc test. F-G. Representative western blotting ( F ) and quantification ( G ) of LC3-II in control and RYR1 patients primary myoblasts starved (EBSS) or incubated with bafilomycin A1 (Baf) for indicated time points. Data are represented as mean + SD *p < 0.05, **p < 0.01, ****p < 0.0001 from two-way ANOVA and Bonferonni post-hoc test. H-I. Confocal imaging of skeletal muscle ( H ) and autophagy index quantification of the normalized RFP to GFP signal ( I ) of larvae co-injected with the autophagic GFP-LC3-RFP-LC3ΔG probe and control-MO or ryr3-MO. *p < 0.05 from Student’s t-test.
Cm10040 Mem Ebss L Glutamine Macgene, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mem+ebss/pm41082335-462-61-73?v=Beijing+Solarbio+Science
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cm10040 mem ebss l glutamine macgene - by Bioz Stars, 2026-07
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minimum essential medium  (CLS Cell Lines Service GmbH)
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CLS Cell Lines Service GmbH minimum essential medium
RyR3 deficiency induces autophagy defects A. Quantification of control and patients primary myoblasts cell death under basal culture conditions and 4h starvation <t>(EBSS).</t> *p < 0.05, ***p < 0.001, ****p < 0.0001, ns , non-significant from 2-way ANOVA and Sidak’s post hoc test. B-C. Representative images ( B ) and quantification ( C ) of LC3 positive vesicles in control and patients’ primary myoblasts cultured in basal or 2h starvation conditions. ****p < 0.0001, ns , non-significan from two-way ANOVA followed by Sidak’s post hoc test. D-E. Representative western blotting ( D ) and quantification ( E ) of LC3-II in control and RYR3 patients’ primary myoblasts starved (EBSS) or incubated with the autophagosome-lysosome fusion <t>inhibitor</t> <t>bafilomycin</t> A1 (Baf) for indicated time points. Data are represented as mean + SD, *p < 0.05, **p < 0.01, ****p < 0.0001 from two-way ANOVA and Bonferonni post-hoc test. F-G. Representative western blotting ( F ) and quantification ( G ) of LC3-II in control and RYR1 patients primary myoblasts starved (EBSS) or incubated with bafilomycin A1 (Baf) for indicated time points. Data are represented as mean + SD *p < 0.05, **p < 0.01, ****p < 0.0001 from two-way ANOVA and Bonferonni post-hoc test. H-I. Confocal imaging of skeletal muscle ( H ) and autophagy index quantification of the normalized RFP to GFP signal ( I ) of larvae co-injected with the autophagic GFP-LC3-RFP-LC3ΔG probe and control-MO or ryr3-MO. *p < 0.05 from Student’s t-test.
Minimum Essential Medium, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mem+ebss/pm41075750-115-14-19?v=CLS+Cell+Lines+Service+GmbH
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minimum essential medium - by Bioz Stars, 2026-07
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mem ebss media  (Cytiva Europe)
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Cytiva Europe mem ebss media
RyR3 deficiency induces autophagy defects A. Quantification of control and patients primary myoblasts cell death under basal culture conditions and 4h starvation <t>(EBSS).</t> *p < 0.05, ***p < 0.001, ****p < 0.0001, ns , non-significant from 2-way ANOVA and Sidak’s post hoc test. B-C. Representative images ( B ) and quantification ( C ) of LC3 positive vesicles in control and patients’ primary myoblasts cultured in basal or 2h starvation conditions. ****p < 0.0001, ns , non-significan from two-way ANOVA followed by Sidak’s post hoc test. D-E. Representative western blotting ( D ) and quantification ( E ) of LC3-II in control and RYR3 patients’ primary myoblasts starved (EBSS) or incubated with the autophagosome-lysosome fusion <t>inhibitor</t> <t>bafilomycin</t> A1 (Baf) for indicated time points. Data are represented as mean + SD, *p < 0.05, **p < 0.01, ****p < 0.0001 from two-way ANOVA and Bonferonni post-hoc test. F-G. Representative western blotting ( F ) and quantification ( G ) of LC3-II in control and RYR1 patients primary myoblasts starved (EBSS) or incubated with bafilomycin A1 (Baf) for indicated time points. Data are represented as mean + SD *p < 0.05, **p < 0.01, ****p < 0.0001 from two-way ANOVA and Bonferonni post-hoc test. H-I. Confocal imaging of skeletal muscle ( H ) and autophagy index quantification of the normalized RFP to GFP signal ( I ) of larvae co-injected with the autophagic GFP-LC3-RFP-LC3ΔG probe and control-MO or ryr3-MO. *p < 0.05 from Student’s t-test.
Mem Ebss Media, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mem+ebss/pmc12273755-57-0-3?v=Cytiva+Europe
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minimal essential medium (mem) with earle’s balanced salt solution (ebss) and l-glutamine (2.0)  (Danaher Inc)
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Danaher Inc minimal essential medium (mem) with earle’s balanced salt solution (ebss) and l-glutamine (2.0)
RyR3 deficiency induces autophagy defects A. Quantification of control and patients primary myoblasts cell death under basal culture conditions and 4h starvation <t>(EBSS).</t> *p < 0.05, ***p < 0.001, ****p < 0.0001, ns , non-significant from 2-way ANOVA and Sidak’s post hoc test. B-C. Representative images ( B ) and quantification ( C ) of LC3 positive vesicles in control and patients’ primary myoblasts cultured in basal or 2h starvation conditions. ****p < 0.0001, ns , non-significan from two-way ANOVA followed by Sidak’s post hoc test. D-E. Representative western blotting ( D ) and quantification ( E ) of LC3-II in control and RYR3 patients’ primary myoblasts starved (EBSS) or incubated with the autophagosome-lysosome fusion <t>inhibitor</t> <t>bafilomycin</t> A1 (Baf) for indicated time points. Data are represented as mean + SD, *p < 0.05, **p < 0.01, ****p < 0.0001 from two-way ANOVA and Bonferonni post-hoc test. F-G. Representative western blotting ( F ) and quantification ( G ) of LC3-II in control and RYR1 patients primary myoblasts starved (EBSS) or incubated with bafilomycin A1 (Baf) for indicated time points. Data are represented as mean + SD *p < 0.05, **p < 0.01, ****p < 0.0001 from two-way ANOVA and Bonferonni post-hoc test. H-I. Confocal imaging of skeletal muscle ( H ) and autophagy index quantification of the normalized RFP to GFP signal ( I ) of larvae co-injected with the autophagic GFP-LC3-RFP-LC3ΔG probe and control-MO or ryr3-MO. *p < 0.05 from Student’s t-test.
Minimal Essential Medium (Mem) With Earle’s Balanced Salt Solution (Ebss) And L Glutamine (2.0), supplied by Danaher Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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minimal essential medium (mem) with earle’s balanced salt solution (ebss) and l-glutamine (2.0) - by Bioz Stars, 2026-07
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Image Search Results


RyR3 deficiency induces autophagy defects A. Quantification of control and patients primary myoblasts cell death under basal culture conditions and 4h starvation (EBSS). *p < 0.05, ***p < 0.001, ****p < 0.0001, ns , non-significant from 2-way ANOVA and Sidak’s post hoc test. B-C. Representative images ( B ) and quantification ( C ) of LC3 positive vesicles in control and patients’ primary myoblasts cultured in basal or 2h starvation conditions. ****p < 0.0001, ns , non-significan from two-way ANOVA followed by Sidak’s post hoc test. D-E. Representative western blotting ( D ) and quantification ( E ) of LC3-II in control and RYR3 patients’ primary myoblasts starved (EBSS) or incubated with the autophagosome-lysosome fusion inhibitor bafilomycin A1 (Baf) for indicated time points. Data are represented as mean + SD, *p < 0.05, **p < 0.01, ****p < 0.0001 from two-way ANOVA and Bonferonni post-hoc test. F-G. Representative western blotting ( F ) and quantification ( G ) of LC3-II in control and RYR1 patients primary myoblasts starved (EBSS) or incubated with bafilomycin A1 (Baf) for indicated time points. Data are represented as mean + SD *p < 0.05, **p < 0.01, ****p < 0.0001 from two-way ANOVA and Bonferonni post-hoc test. H-I. Confocal imaging of skeletal muscle ( H ) and autophagy index quantification of the normalized RFP to GFP signal ( I ) of larvae co-injected with the autophagic GFP-LC3-RFP-LC3ΔG probe and control-MO or ryr3-MO. *p < 0.05 from Student’s t-test.

Journal: medRxiv

Article Title: Novel variants in ryanodine receptor type 3 predispose to acute rhabdomyolysis due to impaired autophagy

doi: 10.64898/2026.02.27.26345848

Figure Lengend Snippet: RyR3 deficiency induces autophagy defects A. Quantification of control and patients primary myoblasts cell death under basal culture conditions and 4h starvation (EBSS). *p < 0.05, ***p < 0.001, ****p < 0.0001, ns , non-significant from 2-way ANOVA and Sidak’s post hoc test. B-C. Representative images ( B ) and quantification ( C ) of LC3 positive vesicles in control and patients’ primary myoblasts cultured in basal or 2h starvation conditions. ****p < 0.0001, ns , non-significan from two-way ANOVA followed by Sidak’s post hoc test. D-E. Representative western blotting ( D ) and quantification ( E ) of LC3-II in control and RYR3 patients’ primary myoblasts starved (EBSS) or incubated with the autophagosome-lysosome fusion inhibitor bafilomycin A1 (Baf) for indicated time points. Data are represented as mean + SD, *p < 0.05, **p < 0.01, ****p < 0.0001 from two-way ANOVA and Bonferonni post-hoc test. F-G. Representative western blotting ( F ) and quantification ( G ) of LC3-II in control and RYR1 patients primary myoblasts starved (EBSS) or incubated with bafilomycin A1 (Baf) for indicated time points. Data are represented as mean + SD *p < 0.05, **p < 0.01, ****p < 0.0001 from two-way ANOVA and Bonferonni post-hoc test. H-I. Confocal imaging of skeletal muscle ( H ) and autophagy index quantification of the normalized RFP to GFP signal ( I ) of larvae co-injected with the autophagic GFP-LC3-RFP-LC3ΔG probe and control-MO or ryr3-MO. *p < 0.05 from Student’s t-test.

Article Snippet: To mimic environmental stress, the GM was replaced with EBSS medium with or without 100 nM bafilomycin A1 (ref: tlrl-bafA1, InvivoGen) for 1h and 3h.

Techniques: Control, Cell Culture, Western Blot, Incubation, Imaging, Injection

A. Representative immunoblot of LC3 lipidation upon starvation (EBSS: Earle’s Balanced Salt Solution) and Bafilomycin A1 treatment (Baf) in primary myoblasts from RYR3 P1 and RYR3 P2 patients. Due to limited proliferative capacity of primary cells, the experiment could only be repeated twice. GM: Growth Medium. β-actin was used as a loading control. n.r. (non relevant) indicates lanes that were included on the blot but are not relevant for the present study. B. Representative immunoblot of LC3 lipidation upon starvation in siRNA-mediated RYR3 depleted primary myoblasts. β-actin was used as a loading control.

Journal: medRxiv

Article Title: Novel variants in ryanodine receptor type 3 predispose to acute rhabdomyolysis due to impaired autophagy

doi: 10.64898/2026.02.27.26345848

Figure Lengend Snippet: A. Representative immunoblot of LC3 lipidation upon starvation (EBSS: Earle’s Balanced Salt Solution) and Bafilomycin A1 treatment (Baf) in primary myoblasts from RYR3 P1 and RYR3 P2 patients. Due to limited proliferative capacity of primary cells, the experiment could only be repeated twice. GM: Growth Medium. β-actin was used as a loading control. n.r. (non relevant) indicates lanes that were included on the blot but are not relevant for the present study. B. Representative immunoblot of LC3 lipidation upon starvation in siRNA-mediated RYR3 depleted primary myoblasts. β-actin was used as a loading control.

Article Snippet: To mimic environmental stress, the GM was replaced with EBSS medium with or without 100 nM bafilomycin A1 (ref: tlrl-bafA1, InvivoGen) for 1h and 3h.

Techniques: Western Blot, Control